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br Materials and methods br Results br Discussion In
2021-06-09
Materials and methods Results Discussion In general, CI symptoms are often visible only after the chilled products have been removed from coolstore to a warmer temperature (Schirra and Cohen, 1999). However, in the present work, 'satsuma’ mandarin fruit stored at 2°C showed chilling symptom
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br Preparation of Recombinant Proteins br Activity
2021-06-09
Preparation of Recombinant Proteins Activity Assays The following solvents are used in the procedures described in this section: Data Analysis Aryl radical rearrangements typically result in a large number of products being generated by Bmp7. Approaches combining mass spectrometry and NMR
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Previously to detect CDKL activity we used a
2021-06-09
Previously, to detect CDKL5 activity, we used a radioactive phosphorylation assay with amphiphysin 1 protein as a substrate [,,]. However, it is necessary to establish a safe and rapid method for analyzing CDKL5 mutant activity. Here, we report a new method for investigating autophosphorylation acti
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While several C terminal peptides have been reported
2021-06-09
While several C-terminal peptides have been reported to have modest (astressin) or substantial (astressin2-B, antisauvagine-30) subtype selectivity for the CRF2 receptor (), [125I]YP20 is the first reported small analog of the C-terminus of CRF exhibiting subtype selectivity for the CRF1 receptor vs
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acalabrutinib Cancer has been linked to both genetic and epi
2021-06-09
Cancer has been linked to both genetic and epigenetic changes. Epigenetic modifications, mainly DNA methylation at CpG dinucleotides, affect the regulation of gene transcription without altering the gene's sequence. Short sequences rich in CpG dinucleotides (i.e., CpG islands) are often found in the
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Here we show that cpg
2021-06-09
Here, we show that cpg2 is expressed only in the FK228 mg and encodes a protein that localizes specifically to the postsynaptic endocytic zone of excitatory synapses. We present evidence that CPG2 is a critical component of the postsynaptic endocytic pathway that mediates both constitutive and acti
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br Outcome of COX overexpression Although a
2021-06-09
Outcome of COX-2 overexpression Although a causal role for COX-2 has been proposed, mechanisms by which COX-2 function contributes to the pathogenesis of hyperplastic disease are not well defined. To examine if there is any correlation between COX-2 and p53 protein levels, Kumagai et al. [15] rec
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COMT expression protein level and activity are down regulate
2021-06-09
COMT expression, protein level and activity are down regulated by E2. Using estrogen receptor (ER) positive MCF-7 cells, Xie et al. demonstrated an E2 dose dependent (1, 10 and 100 nM) decrease in S-COMT 1.3 kb mRNA that was maximum after 48 h (Xie et al., 1999). A similar decrease was not observed
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Due to its several industrial applications the
2021-06-09
Due to its several industrial applications, the recombinant production of collagen and all its derivatives as gelatins has been pursued for many years in different biological systems as mammalian 14520 milliliters to liters (Toman et al., 1999), tobacco plants (Ruggiero et al., 2000), silkworms (To
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NPC L expression is both tissue and species specific Consist
2021-06-09
NPC1L1 expression is both tissue- and species-specific. Consistent with its function, NPC1L1 is mainly localized to the brush border membrane of enterocytes and the canalicular membrane of hepatocytes. NPC1L1, which is expressed in the liver of humans and rats but not mice, mediates cholesterol ochr
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suramin The genetic basis of resistance to dieldrin
2021-06-09
The genetic basis of resistance to dieldrin has been described for several insect species as a mutation in the transmembrane domain II of the GABA-Cl or rdl (resistance to dieldrin) gene (ffrench-Constant et al., 2004; Bass et al., 2004; Du et al., 2005). A mutation in the GABA-Cl gene was found at
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br Effects of estrogen on vascular
2021-06-09
Effects of estrogen on vascular function Atherosclerosis is a chronic inflammatory condition of the vascular wall (Figure 1) that can be converted to an acute clinical event by the induction of plaque rupture or erosion, leading to thrombosis [58]. This perpetuating process is characterized by ph
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Even minor alterations in the surfactants structure
2021-06-09
Even minor alterations in the surfactants structure could have consequences on their physico-chemical characteristics; mono-RLs have less solubility, stronger surfaces sorb, and stronger cationic metals binding than homologue di-RLs (Zhang et al., 1997). The HPLC separation of RLs structure is base
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Neutrophils produce high amounts of ROS which act as
2021-06-09
Neutrophils produce high amounts of ROS, which act as antimicrobial agents, signaling molecules or initiators during PMA-induced NET formation. Of the intravenous anesthetics used in this study, propofol was the best at decreasing PMA-induced ROS levels (Fig. 3A and B). Our previous study also demon
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Another interesting finding regarding substrate selectivity
2021-06-09
Another interesting finding regarding substrate selectivity of iPLA2-VIA in whole cellular systems stems from the observation that some of the major species hydrolyzed by the enzyme contain a 16:1 fatty abk at the sn-2 position [57,119], raising the possibility that iPLA2-VIA may constitute a major
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